The protein has a predicted molecular mass of 30.2 kDa after removal of the signal peptide. The apparent molecular mass of CCR8-mFc is approximately 33-55 kDa due to glycosylation.
Conjugate
Unconjugated
Purity
> 95% by SDS-PAGE and Coomassie blue staining.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Reconstitution
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
The mouse Fc tag consists of amino acids Pro99-Lys330.
ELISA plates were pre-coated with 2 µg/ml (100 µl/well) Recombinant Human CCR8 Protein (Fc Tag) (A317861) which can bind Anti-CCR8 Humanized Antibody [10A11] - Azide free (A318895) in a linear range of 1.37-111.11 µg/ml.
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