Lyophilized from Phosphate Buffered Saline, pH 7.4, with 8% Trehalose (0.22µm filter sterilized).
Endotoxin Level
<1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Standard Curve - Recombinant Human CD116 Protein (C-terminal His Tag) (A330221)
ELISA examination of immobilized Human GM-CSF, No Tag, (5 µg/mL) binding Recombinant Human CD116 Protein (C-terminal His Tag) (A330221): EC50 =1.0 µg/mL.
Standard Curve - Recombinant Human CD116 Protein (C-terminal His Tag) (A330221)
ELISA examination of immobilized Human GM-CSF R alpha, His Tag, (1 µg/mL) binding Recombinant Human CD116 Protein (C-terminal His Tag) (A330221): EC50 =6.6ng/mL.
Recombinant Human CD116 Protein (C-terminal His Tag) (A330221)
Recombinant Human CD116 Protein (C-terminal His Tag) (A330221) binding to Human GM-CSF R alpha (His Tag) on a CM5 Chip via anti-His antibody, analyzed by surface plasmon resonance (SPR; Biacore T200). Affinity constant = 0.79nM.
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