Immobilized Recombinant Human IL7 (2 µg/mL) bind Recombinant Human IL7R alpha in a functional ELISA: EC50 = 48.44 ng/mL. Immobilized CD127 Mouse mAb (1µg/mL (25 µL/well) can bind Human CD34 in a functional ELISA with a linear range of 0.46-5.89ng/mL.
Purity
>95% (by SDS-PAGE).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).
Endotoxin Level
<1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Standard Curve - Recombinant Human CD127 Protein (C-terminal Human Fc and His Tag) (A330222)
ELISA examination of immobilized Recombinant Human IL7 (2 µg/mL) binding Recombinant Human CD127 Protein (C-terminal Human Fc and His Tag) (A330222): EC50 = 48.44 ng/mL.
Standard Curve - Recombinant Human CD127 Protein (C-terminal Human Fc and His Tag) (A330222)
ELISA examination of immobilized CD127 Mouse mAb (1µg/mL) binding Recombinant Human CD127 Protein (C-terminal Human Fc and His Tag) (A330222): linear range = 0.46-5.89ng/mL.
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