Lyophilized from Phosphate Buffered Saline, pH 7.4, with 8% Trehalose (0.22µm filter sterilized).
Endotoxin Level
<1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Recombinant Human CD3 + CD3d Protein (C-terminal His Tag) (A330302)
Tris-Bis PAGE of Recombinant Human CD3 + CD3d Protein (C-terminal His Tag) (A330302) under reducing conditions confirms that purity is greater than 95%.
Standard Curve - Recombinant Human CD3 + CD3d Protein (C-terminal His Tag) (A330302)
ELISA examination of immobilized Human CD3E and CD3D at 2 µg/mL) binding Recombinant Human CD3 + CD3d Protein (C-terminal His Tag) (A330302): EC50 =18.9ng/mL.
Standard Curve - Recombinant Human CD3 + CD3d Protein (C-terminal His Tag) (A330302)
ELISA examination of immobilized Human CD3E and CD3D (2ug/mL) binding Recombinant Human CD3 + CD3d Protein (C-terminal His Tag) (A330302): EC50 =18.5ng/mL.
Recombinant Human CD3 + CD3d Protein (C-terminal His Tag) (A330302)
OKT3 (mFc Tag) binding to Recombinant Human CD3 + CD3d Protein (C-terminal His Tag) (A330302) on a CM5 Chip via Anti-His Antibody, analyzed by surface plasmon resonance (SPR; Biacore T200). Affinity constant = 0.36nM.
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