Recombinant Human CD40 Protein (Fc Chimera 6xHis Tag)
Applications
ELISA, SDS-PAGE
Expression System
HEK293 cells
Nature
Recombinant
Protein Species
Human
Protein Length
Protein fragment.
Sequence
CD40 (Glu21-Arg193) + mFc (Pro99-Lys330) + 6xHis Tag
Tag
C-terminal Mouse Fc Tag and 6xHis Tag
Molecular Weight
The protein has a predicted molecular mass of 68 kDa after removal of the signal peptide.
Conjugate
Unconjugated
Purity
> 95% by SDS-PAGE and Coomassie blue staining.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Reconstitution
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
The mouse Fc tag consists of amino acids Pro99-Lys330.
Recombinant Human CD40 Protein (Fc Chimera 6xHis Tag) (A318393)
ELISA plates were pre-coated with Recombinant Human CD40 Protein (Fc Chimera 6xHis Tag) (A318393) at 2 µg/ml (100 µl/well) which can bind Iscalimab Biosimilar - Anti-CD40 Antibody - BSA and Azide free (A318936) in a linear range of 0.64-80.0 ng/ml.
Recombinant Human CD40 Protein (Fc Chimera 6xHis Tag) (A318393)
ELISA plates were pre-coated with Recombinant Human TRAP/CD40L Protein (Fc Tag) (A318295) at 2 µg/ml (100 µl/well) which can bind Recombinant Human CD40 Protein (Fc Chimera 6xHis Tag) (A318393) in a linear range of 0.51-320 ng/ml.
Publishing research using Recombinant Human CD40 Protein (Fc Chimera 6xHis Tag) (A318393)? Please let us know so that we can list the citation on this page.
Alternative products to Recombinant Human CD40 Protein (Fc Chimera 6xHis Tag) (A318393)
