Recombinant Human CD47 Protein (C-terminal His Tag) (A330353)

Name: Recombinant Human CD47 Protein (C-terminal His Tag)
Brand: Antibodies.com
SKU: A330353
Price: 130.0 GBP
Availability: InStock

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$180

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Recombinant Human CD47 Protein (C-terminal His Tag)

Expression System

HEK293 cells

Nature

Recombinant

Protein Species

Human

Sequence

QLLFNKTKSVEFTFCNDTVVIPCFVTNMEAQNTTEVYVKWKFKGRDIYTFDGALNKSTVPTDFSSAKIEVSQLLKGDASLKMDKSDAVSHTGNYTCEVTELTREGETIIELKYRVVSWFSP

Tag

C-terminal His Tag

Conjugate

Unconjugated

Biological Activity

Immobilized Human CD47 (5 µg/mL) bind Human SIRPA in a functional ELISA with a linear range of 0.488-189.26 ng/mL. Immobilized Human CD47 (2 µg/mL) bind Human SIRP-alpha/CD172a in a functional ELISA with a linear range of 0.06-22.3 ng/mL. Loaded Human SIRP-alpha/CD172a Protein, C-terminal Human Fc and His Tag, on ProA Biosensor, can bind Human CD47 Protein, C-His Tag, in BLI assay (Gator) with an affinity constant of 11.1 nM.

Purity

>95% (by SDS-PAGE).

Product Form

Lyophilized

Concentration

Reconstitution dependent.

Formulation

Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).

Endotoxin Level

<1 EU/µg of the protein by LAL method.

Storage

Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.

Synonyms

Antigenic surface determinant protein OA3, IAP, Integrin-associated protein, Leukocyte surface antigen CD47, MER6, Protein MER6

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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SDS-PAGE - Recombinant Human CD47 Protein (C-terminal His Tag) (A330353)

SDS-PAGE of Recombinant Human CD47 Protein (C-terminal His Tag) (A330353) using Coomassie Blue to show a band at 35-45 kDa.

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Standard Curve - Recombinant Human CD47 Protein (C-terminal His Tag) (A330353)

ELISA examination of immobilized Human CD47 (5 µg/mL) binding Recombinant Human CD47 Protein (C-terminal His Tag) (A330353): linear range = 0.488-189.26 ng/mL.

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Standard Curve - Recombinant Human CD47 Protein (C-terminal His Tag) (A330353)

ELISA examination of immobilized Human CD47 (2 µg/mL) binding Recombinant Human CD47 Protein (C-terminal His Tag) (A330353): linear range = 0.06-22.3 ng/mL.

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Recombinant Human CD47 Protein (C-terminal His Tag) (A330353)

Recombinant Human CD47 Protein (C-terminal His Tag) (A330353) binding to Human SIRP-alpha/CD172a Protein, C-Human Fc and His Tag, immobilized on ProA Biosensor, analyzed by bio-layer interferometry (BLI; Gator). Affinity constant = 11.1 nM.

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