Recombinant Human CD70 Protein (Fc Tag & 6xHis Tag)
Applications
ELISA, SDS-PAGE
Expression System
HEK293 cells
Nature
Recombinant
Protein Species
Human
Protein Length
Protein fragment.
Sequence
mFc (Pro99-Lys330) + CD70 (Gln39-Pro193) + 6xHis Tag
Tag
N-terminal Mouse Fc Tag and C-terminal 6xHis Tag
Molecular Weight
The protein has a predicted molecular mass of 45.6 kDa after removal of the signal peptide.
Conjugate
Unconjugated
Purity
> 95% by SDS-PAGE and Coomassie blue staining.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Recombinant Human CD70 Protein (Fc Tag & 6xHis Tag) (A318368)
ELISA plates were pre-coated with Recombinant Human CD27 Protein (Fc Tag) (A318253) at 2 µg/ml (100 µl/well) which can bind Recombinant Human CD70 Protein (Fc Tag & 6×His Tag) (A318368) in a linear range of 3.2-400 ng/ml.
Recombinant Human CD70 Protein (Fc Tag & 6xHis Tag) (A318368)
ELISA plates were pre-coated with Recombinant Human CD70 Protein (Fc Tag & 6×His Tag) (A318368) at 2 µg/ml (100 µl/well) which can bind Vorsetuzumab Biosimilar - Anti-CD70 Antibody - BSA and Azide free (A318951) in a linear range of 3.91-139.9 ng/ml.
Flow Cytometry - Recombinant Human CD70 Protein (Fc Tag & 6xHis Tag) (A318368)
Flow cytometry analysis of 15µg/ml of Recombinant Human CD70 Protein (Fc Tag & 6×His Tag) (A318368) on Expi293 cells transfected with human CD27 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
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