Immobilized Human 5-Nucleotidase/CD73 (2 µg/mL) bind 5-Nucleotidase/CD73 Rabbit pAb in a functional ELISA with a linear range of 0.16-2.93 ng/mL. Hydrolysis of the the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP) measured by a Malachite Green Phosphate Detection Kit: Specific activity = >33654 pmol/min/µg. Hydrolysis of the the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP): Specific activity = >25, 000 pmol/min/µg.
Purity
>95% (by SDS-PAGE).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).
Endotoxin Level
<0.1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Standard Curve - Recombinant Human CD73 Protein (C-terminal His Tag) (A330395)
ELISA examination of immobilized Human 5'-Nucleotidase/CD73 (2 µg/mL) binding Recombinant Human CD73 Protein (C-terminal His Tag) (A330395): linear range = 0.16-2.93 ng/mL.
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