Lyophilized from Phosphate Buffered Saline, pH 7.4, with 8% Trehalose (0.22µm filter sterilized).
Endotoxin Level
<1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Recombinant Human CD80 Protein (C-terminal His and Avi Tag) (A330408)
Tris-Bis PAGE of Recombinant Human CD80 Protein (C-terminal His and Avi Tag) (A330408) under reducing conditions confirms that purity is greater than 95%.
Standard Curve - Recombinant Human CD80 Protein (C-terminal His and Avi Tag) (A330408)
ELISA examination of immobilized Human CTLA-4, hFc Tag, (1 µg/mL) binding Recombinant Human CD80 Protein (C-terminal His and Avi Tag) (A330408): EC50 =1.48 µg/mL.
Recombinant Human CD80 Protein (C-terminal His and Avi Tag) (A330408)
Human CTLA4 (hFc Tag) binding to Recombinant Human CD80 Protein (C-terminal His and Avi Tag) (A330408) on a CM5 Chip via Anti-His Antibody, analyzed by surface plasmon resonance (SPR; Biacore T200). Affinity constant = 1.1nM.
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