Immobilized Recombinant Human M-CSF (2 µg/mL) bind Recombinant Human CSF1R in a functional ELISA with a linear range of 50-200 ng/mL. Inhibition of the the GCSF-induced proliferation of M-NFS-60 mouse myelogenous leukemia lymphoblast cells: ED50 = typically 0.095-0.38 ng/mL. Immobilized Human CSF-1/M-CSF (2µg/mL) bind Human CSF1R in a functional ELISA with a linear range of 2-185 ng/mL.
Purity
>95% (by SDS-PAGE).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).
Endotoxin Level
<0.1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Standard Curve - Recombinant Human CSF-1-R Protein (C-terminal His Tag) (A330485)
ELISA examination of immobilized Recombinant Human M-CSF (2µg/mL) binding Recombinant Human CSF-1-R Protein (C-terminal His Tag) (A330485): linear range = 50-200 ng/mL.
Recombinant Human CSF-1-R Protein (C-terminal His Tag) (A330485)
Inhibition of GCSF-induced cell proliferation for M-NFS-60 Mouse myelogenous leukemia lymphoblast cells with Recombinant Human CSF-1-R Protein (C-terminal His Tag) (A330485): ED50 = typically 0.095-0.38 ng/mL.
Standard Curve - Recombinant Human CSF-1-R Protein (C-terminal His Tag) (A330485)
ELISA examination of immobilized Human CSF-1/M-CSF(Cat. RP01221) (2µg/mL) binding Recombinant Human CSF-1-R Protein (C-terminal His Tag) (A330485): linear range = 2-185 ng/mL.
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