Immobilized Human DKK-1 (5 µg/mL) bind Human LRP-5 in a functional ELISA with a linear range of 0.027-0.559 µg/mL. Inhibition of the Wnt3a-induced alkaline phosphatase production by C3H10T1/2 cells: EC50 =approximately 95 ng/ml in the presence of 2 µg/mL of Recombinant Human Wnt3a.
Purity
>95% (by SDS-PAGE).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).
Endotoxin Level
<0.1 EU/µg.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Synonyms
Dickkopf-1, Dickkopf-related protein 1, Dkk-1, hDkk-1, SK
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Standard Curve - Recombinant Human DKK1 Protein (C-terminal His Tag) (A330529)
ELISA examination of immobilized Human DKK-1 (5 µg/mL) binding Recombinant Human DKK1 Protein (C-terminal His Tag) (A330529): linear range = 0.027-0.559 µg/mL.
Standard Curve - Recombinant Human DKK1 Protein (C-terminal His Tag) (A330529)
ELISA examination of Recombinant Human DKK1 Protein (C-terminal His Tag) (A330529) to inhibit Wnt3a-induced alkaline phosphatase production by C3H10T1/2 cells: EC50 = approximately 95 ng/ml in the presence of Recombinant Human Wnt3a (2 µg/mL).
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