Immobilized Recombinant Human TRAIL (2 µg/mL) bind Recombinant Human DR5 in a functional ELISA with a linear range of 14-56 ng/mL. Inhibition of the TRAIL-mediated cytotoxicity using L-929 mouse fibroblast cells treated with TRAIL: ED50 = 0.38-1.5 pg/mL in the presence of 20 ng/mL Recombinant Human TRAIL/TNFSF10. Immobilized Human TRAIL / TNFSF10 Protein (2µg/mL) bind Human CD262 Protein in a functional ELISA with a linear range of 0.5-14.1 ng/mL.
Purity
>90% (by SDS-PAGE).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).
Endotoxin Level
<0.1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Standard Curve - Recombinant Human DR5 Protein (C-terminal His Tag) (A330549)
ELISA examination of immobilized Recombinant Human TRAIL (2µg/mL) binding Recombinant Human DR5 Protein (C-terminal His Tag) (A330549): linear range = 14-56 ng/mL.
Recombinant Human DR5 Protein (C-terminal His Tag) (A330549)
TRAIL-mediated cytotoxicityin L-929 mouse fibroblast cells treated with TRAIL inhibited by Recombinant Human DR5 Protein (C-terminal His Tag) (A330549): ED50 = 0.38-1.5 pg/mL in the presence of 20 ng/mL Recombinant Human TRAIL/TNFSF10.
Standard Curve - Recombinant Human DR5 Protein (C-terminal His Tag) (A330549)
ELISA examination of immobilized Human TRAIL / TNFSF10 Protein (2µg/mL) binding Recombinant Human DR5 Protein (C-terminal His Tag) (A330549): linear range = 0.5-14.1 ng/mL.
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