Recombinant Human E Cadherin Protein (C-terminal His Tag) (A330552)

Name: Recombinant Human E Cadherin Protein (C-terminal His Tag)
Brand: Antibodies.com
SKU: A330552
Price: 95.0 GBP
Availability: InStock

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$140

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Recombinant Human E Cadherin Protein (C-terminal His Tag)

Expression System

HEK293 cells

Nature

Recombinant

Protein Species

Human

Sequence

DWVIPPISCPENEKGPFPKNLVQIKSNKDKEGKVFYSITGQGADTPPVGVFIIERETGWLKVTEPLDRERIATYTLFSHAVSSNGNAVEDPMEILITVTDQNDNKPEFTQEVFKGSVMEGALPGTSVMEVTATDADDDVNTYNAAIAYTILSQDPELPDKNMFTINRNTGVISVVTTGLDRESFPTYTLVVQAADLQGEGLSTTATAVITVTDTNDNPPIFNPTTYKGQVPENEANVVITTLKVTDADAPNTPAWEAVYTILNDDGGQFVVTTNPVNNDGILKTAKGLDFEAKQQYILHVAVTNVVPFEVSLTTSTATVTVDVLDVNEAPIFVPPEKRVEVSEDFGVGQEITSYTAQEPDTFMEQKITYRIWRDTANWLEINPDTGAISTRAELDREDFEHVKNSTYTALIIATDNGSPVATGTGTLLLILSDVNDNAPIPEPRTIFFCERNPKPQVINIIDADLPPNTSPFTAELTHGASANWTIQYNDPTQESIILKPKMALEVGDYKINLKLMDNQNKDQVTTLEVSVCDCEGAAGVCRKAQPVEAGLQIPA

Tag

C-terminal His Tag

Conjugate

Unconjugated

Biological Activity

Immobilized Human CDH1 (1 µg/mL) bind Human CTNNB1 in a functional ELISA with a linear range of 0.1-0.78 µg/mL. Immobilized protein to support the adhesion of MCF-7 human breast adenocarcinoma cells when added to E-Cad coated plates (5 µg/mL, 100 µL/well): >12 % will adhere specifically after 90 minutes (37°C).

Purity

>95% (by SDS-PAGE).

Product Form

Lyophilized

Concentration

Reconstitution dependent.

Formulation

Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).

Endotoxin Level

<0.1 EU/µg of the protein by LAL method.

Storage

Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.

Synonyms

Arc 1, CADH1_HUMAN, Cadherin 1, cadherin 1 type 1 E-cadherin, Cadherin1, CAM 120/80, CD 234, CD 324, CD324, CD324 antigen, CDH1, CDHE, E-Cad/CTF3, E-cadherin, ECAD , Epithelial cadherin, epithelial calcium dependant adhesion protein, LCAM, Liver cell adhesion molecule, UVO, Uvomorulin

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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SDS-PAGE - Recombinant Human E Cadherin Protein (C-terminal His Tag) (A330552)

SDS-PAGE of Recombinant Human E Cadherin Protein (C-terminal His Tag) (A330552) using Coomassie Blue to show a band at 70-85kDa.

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Standard Curve - Recombinant Human E Cadherin Protein (C-terminal His Tag) (A330552)

ELISA examination of immobilized recombinant Human CDH1 (1 µg/mL) binding Recombinant Human E Cadherin Protein (C-terminal His Tag) (A330552): linear range = 0.1-0.78µg/mL.

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