Stimulation of the EGF Receptor autophosphorylation in A431 cells: 1-10 ng/mL. Immobilized Human EGFR at 5 µg/mL bind Human EGF in a functional ELISA with a linear range of 7-25 ng/mL. Cell proliferation assay using BALB/c 3T3 mouse embryonic fibroblasts: ED50 = typically 0.065-0.26 ng/mL; Specific activity =3.84×10^6-1.54×10^7units/mg.
Purity
>90% (by SDS-PAGE).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).
Endotoxin Level
<0.1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Synonyms
Pro-epidermal growth factor
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Recombinant Human EGF Protein (C-terminal Human Fc and His Tag) (A330555)
EGF Receptor autophosphorylation in A431 cells analyzed by western blot. Primary antibody incubation was performed with Recombinant Human EGF Protein (C-terminal Human Fc and His Tag) (A330555) at 1-10 ng/mL and detected with chemiluminescence.
Standard Curve - Recombinant Human EGF Protein (C-terminal Human Fc and His Tag) (A330555)
ELISA examination of immobilized Human EGFR (5 µg/mL) binding Recombinant Human EGF Protein (C-terminal Human Fc and His Tag) (A330555): linear range = 7-25ng/mL.
Recombinant Human EGF Protein (C-terminal Human Fc and His Tag) (A330555)
Cell proliferation assay using Recombinant Human EGF Protein (C-terminal Human Fc and His Tag) (A330555) on BALB/c 3T3 Mouse embryonic fibroblasts cells : ED50 = typically 0.065-0.26 ng/mL; Specific activity = 3.84×10^6-1.54×10^7units/mg.
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