Ability to induce apoptosis of Jurkat human acute T cell leukemia cells: ED50 = 2.075-8.3ng/mL in the presence of 10 µg/mL of a cross-linking antibody Rabbit Anti-poly Histidine Monoclonal Antibody ; Specific activity = 1.20×10^5-4.82×10^5 units/mg.
Purity
>95% (by Tris-Bis PAGE).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).
Reconstitution
Centrifuge the vial before opening and reconstitute with distilled sterile water to a concentration of 0.1-0.5 mg/mL. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer.
Endotoxin Level
<1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Recombinant Human Fas Ligand Protein (N-terminal His Tag) (A330613)
Recombinant Human Fas Ligand Protein (N-terminal His Tag) (A330613) induce apoptosis of Jurkat cells: ED50 = 2.075-8.3 ng/mL in the presence of 10 µg/mL of a cross-linking antibody Rabbit Anti-poly Histidine Monoclonal Antibody; Specific activity = 1.20×10^5~4.82×10^5 units/mg.
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