Immobilized recombinant Human Fas Ligand (2 µg/mL) bind recombinant Human FAS. The EC50 of Human FAS is 6.23 ng/mL. Inhibition of the Fas Ligand-induced apoptosis of Jurkat Human acute T cell leukemia cells: ED50 = typically 16.5-66 ng/mL in the presence of 5 ng/mL Recombinant Human Fas Ligand.
Purity
>90% (by SDS-PAGE).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).
Endotoxin Level
<0.1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Standard Curve - Recombinant Human Fas Protein (C-terminal Human Fc and His Tag) (A330612)
ELISA examination of immobilized recombinant Human Fas Ligand (2µg/mL) binding Recombinant Human Fas Protein (C-terminal Human Fc and His Tag) (A330612): EC50 = 6.23 ng/mL.
Recombinant Human Fas Protein (C-terminal Human Fc and His Tag) (A330612)
Inhibition of Fas Ligand-induced apoptosis of Jurkat Human acute T cell leukemia cells with Recombinant Human Fas Protein (C-terminal Human Fc and His Tag) (A330612): ED50 = typically 16.5-66 ng/mL in the presence of 5 ng/mL Recombinant Human Fas Ligand.
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