Immobilized Recombinant Human FGF1 (5 µg/mL) bind Recombinant Human FGFR3 in a functional ELISA with a linear range of 0.5-1.5 µg/mL. Cell proliferation assay using BALB/c 3T3 mouse embryonic fibroblasts: ED50 = typically 22-140 pg/mL.
Purity
>95% (by SDS-PAGE).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from 50mM MOPS, pH 7.9, with 100mM Na2SO4 and 10mM EDTA (0.22µm filter sterilized).
Endotoxin Level
<1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Standard Curve - Recombinant Human FGF1 Protein (C-terminal His Tag) (A330621)
ELISA examination of immobilized Recombinant Human FGF1 (5µg/mL) binding Recombinant Human FGF1 Protein (C-terminal His Tag) (A330621): linear range = 0.5-1.5 µg/mL.
Recombinant Human FGF1 Protein (C-terminal His Tag) (A330621)
Cell proliferation assay using Recombinant Human FGF1 Protein (C-terminal His Tag) (A330621) on BALB/c 3T3 Mouse embryonic fibroblasts cells : ED50 = typically 22-140 pg/mL.
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