Immobilized Human FGF2 (0.5 µg/mL) bind Human GPC3 in a functional ELISA with a linear range of 7-20 ng/mL. Cell proliferation assay using BALB/c 3T3 mouse embryonic fibroblasts: ED50 = typically 0.635-2.54 ng/mL; Specific activity =3.94 × 10^5~1.57 × 10^6 units/mg. Recombinant Human VEGFA(40 ng/mL) and bFGF (50 ng/mL) induce mesoderm cells to differentiate into hematopoietic stem and progenitor cells: pebbly-like CD43+ hematopoietic stem and progenitor cells appeared in the hematogenic endothelium after 4 days. Primary neural stem cells cultured with bFGF (20 ng/mL): particle size of the suspended neural stem cells gradually increased.
Purity
>95% (by SDS-PAGE).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from 20mM Tris, pH 7.5, with 150mM Nacl (0.22µm filter sterilized).
Endotoxin Level
<1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Cell proliferation assay using Recombinant Human FGF2 Protein (A330626) on Balb3T3 Mouse embryonic fibroblasts cells : ED50 = 0.05-0.21 ng/mL; Specific activity = 4.76×10^6~2.00×10^7 units/mg.
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