Inhibition of the FGF-acidic dependent proliferation of Balb/c 3T3 mouse fibroblasts: ED50 = typically 0.21-0.84 ng/mL; Specific activity =1.19×10^6-4.76×10^6units/mg. Immobilized Recombinant Human FGFR2 at 2 µg/mL bind Recombinant Human FGF1 in a functional ELISA with a linear range of 0.128-48.3 ng/mL.
Purity
>97% (by SDS-PAGE).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from 20mM Tris, pH 8.0, with 200mM NaCl (0.22µm filter sterilized).
Endotoxin Level
<1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Inhibition of FGF-acidic dependent cell proliferation for Balb/c 3T3 mouse fibroblast cells with Recombinant Human FGFR2 Protein (A330635): ED50 = typically 0.21-0.84 ng/mL; Specific activity = 1.19×10^6-4.76×10^6units/mg.
