Immobilized recombinant human FGF2 (1 µg/mL) bind recombinant human FGFR4 in a functional ELISA with a linear range of 30-125 ng/mL. Immobilized recombinant human FGFR4 (5 µg/mL) bind recombinant human FGF12 in a functional ELISA with a linear range of 35-100 ng/mL. Inhibition of the FGF-acidic dependent proliferation of Balb/c 3T3 mouse fibroblasts: ED50 = 0.03-0.12 ng/mL.
Purity
>95% (by SDS-PAGE).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).
Endotoxin Level
<0.1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Standard Curve - Recombinant Human FGFR4 Protein (C-terminal Human Fc and His Tag) (A330653)
ELISA examination of immobilized recombinant human FGF2 (1 µg/mL) binding Recombinant Human FGFR4 Protein (C-terminal Human Fc and His Tag) (A330653): linear range = 30-125 ng/mL.
Standard Curve - Recombinant Human FGFR4 Protein (C-terminal Human Fc and His Tag) (A330653)
ELISA examination of immobilized recombinant human FGFR4 (5µg/mL) binding Recombinant Human FGFR4 Protein (C-terminal Human Fc and His Tag) (A330653): linear range = 35-100ng/mL.
Recombinant Human FGFR4 Protein (C-terminal Human Fc and His Tag) (A330653)
Inhibition of FGF-acidic dependent proliferation for Balb/c 3T3 mouse fibroblasts with Recombinant Human FGFR4 Protein (C-terminal Human Fc and His Tag) (A330653): ED50 = typically 0.03-0.12 ng/mL.
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