ED50: <0.07 ng/ml, as determined by the dose-dependant proliferation of murine NFS-60 indicator cells (measured by 3H-thymidine uptake), corresponding to a Specific Activity of 1.27 x 108 IU/mg.
Purity
>97% as determined by SDS-PAGE.
Product Form
Lyophilized
Concentration
1 mg/ml
Formulation
Lyophilized from a solution containing 10 mM Hydrochloric Acid, pH 6.5, with 0.4 mg Tween 20, 100 mg Mannitol, 160 mg L-Arginine, 40 mg Phenylalanine, and 4 mg Methionine.
Storage
Shipped at ambient temperature. Lyophilized: Short term (3 weeks) store at 25°C. Long term store desiccated below -18°C. Reconstituted: Short term (2-7 days) store at 4°C. Long term store below -18°C, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid freeze/thaw cycles.