Recombinant Human Glypican 3 Protein (Biotin) (C-terminal His and Avi Tag)
Expression System
HEK293 cells
Nature
Recombinant
Protein Species
Human
Tag
C-terminal His and Avi Tag
Conjugate
Biotin
Purity
>95% (by Tris-Bis PAGE).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).
Endotoxin Level
<1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Synonyms
Glypican-3, GPC3, GTR2-2, Intestinal protein OCI-5, MXR7, OCI5
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Recombinant Human Glypican 3 Protein (Biotin) (C-terminal His and Avi Tag) (A330710)
Tris-Bis PAGE of Recombinant Human Glypican 3 Protein (Biotin) (C-terminal His and Avi Tag) (A330710) under reducing conditions confirms that purity is greater than 90%.
Flow Cytometry - Recombinant Human Glypican 3 Protein (Biotin) (C-terminal His and Avi Tag) (A330710)
Expression rate of Anti-GPC3-CAR positive cells detected by Recombinant Human Glypican 3 Protein (Biotin) (C-terminal His and Avi Tag) (A330710). 293T cells and anti-GPC3 CAR-293T cells incubated with Recombinant Human Glypican 3 Protein (Biotin) (C-terminal His and Avi Tag) (A330710) and Biotinylated Protein Control-His. Negative control: non-transfected 293 cells and Biotinylated Protein Control-His. SA-FITC used to evaluate the binding activity of Recombinant Human Glypican 3 Protein (Biotin) (C-terminal His and Avi Tag) (A330710).
Standard Curve - Recombinant Human Glypican 3 Protein (Biotin) (C-terminal His and Avi Tag) (A330710)
ELISA examination of immobilized Anti-GPC3 Antibody, hFc Tag, (0.5µg/mL) binding Recombinant Human Glypican 3 Protein (Biotin) (C-terminal His and Avi Tag) (A330710): EC50 =10.8ng/ml.
Flow Cytometry - Recombinant Human Glypican 3 Protein (Biotin) (C-terminal His and Avi Tag) (A330710)
Expression rate of Anti-GPC3-CAR positive cells detected by Recombinant Human Glypican 3 Protein (Biotin) (C-terminal His and Avi Tag) (A330710). 293T cells and anti-GPC3 CAR-293T cells incubated with Recombinant Human Glypican 3 Protein (Biotin) (C-terminal His and Avi Tag) (A330710) and Biotinylated Protein Control-His. Negative control: non-transfected 293 cells and Biotinylated Protein Control-His. SA-FITC used to evaluate the binding activity of Recombinant Human Glypican 3 Protein (Biotin) (C-terminal His and Avi Tag) (A330710).
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Proteins predicted to interact with Glypican 3
Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
