The protein has a predicted molecular mass of 31.1 kDa after removal of the signal peptide. The apparent molecular mass of GPR75-mFc is approximately 35-55 kDa due to glycosylation.
Conjugate
Unconjugated
Purity
> 95% by SDS-PAGE and Coomassie blue staining.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Reconstitution
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
The mouse Fc tag consists of amino acids Pro99-Lys330.
Synonyms
G protein coupled receptor 75, GPR chr2, Gpr75, GPR75_HUMAN, GPRchr2, OTTHUMP00000159608, Probable G protein coupled receptor 75, Probable G-protein coupled receptor 75, WI 31133, WI31133
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Recombinant Human GPCR GPR75 Protein (Fc Tag) (A317700)
ELISA plates were pre-coated with Recombinant Human GPCR GPR75 Protein (Fc Tag) (A317700) at 2 µg/ml (100 µl/well) which can bind Anti-GPCR GPR75 Antibody [DMC368] - BSA and Azide free (A318765) in a linear range of 16-400 ng/ml.
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