Protease activity on HtrA2 (0.02 mg/mL) cleavage of bovine ß-casein (0.2 mg/mL) by SDS-PAGE: >95% cleavage of ß-casein, after incubation for 60 minutes at 45? in 50 mM Tris, pH 8.0.
Purity
>92% (by SDS-PAGE).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from 20mM Tris, pH 8.0, with 150mM NaCl (0.22µm filter sterilized).
Reconstitution
Centrifuge the vial before opening and reconstitute with distilled sterile water to a concentration of 0.1-0.5 mg/mL. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Synonyms
High temperature requirement protein A2, OMI, Omi stress-regulated endoprotease, PRSS25, Serine protease 25, Serine protease HTRA2, mitochondrial, Serine proteinase OMI
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
