Immobilized Human FNGR1/CD119 (1 µg/mL) bind Human IFNG in a functional ELISA with a linear range of 0.98-1.97 ng/mL. Cytotoxicity assay using HT-29 cells: ED50 = 0.74-2.96 ng/mL; Specific activity =3.38×10^5~1.35×10^6 units/mg.
Purity
>95% (by SDS-PAGE).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).
Endotoxin Level
<0.1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Cytotoxicity effects induced by Recombinant Human IFN gamma Protein (A330762) in HT-29 cells: ED50 = 0.74-2.96 ng/mL; Specific activity = 3.38×10^5~1.35×10^6 units/mg.
Flow Cytometry - Recombinant Human IFN gamma Protein (A330762)
Flow cytometry analysis of A549 cells (1X10^6) treated with Recombinant Human IFN gamma Protein (A330762) at 1 ng/mL stained with PE Rabbit IgG isotype control at 5 µl/Test (left) or PE Rabbit anti-Human PD-L1/CD274 mAb at 5 µl/Test (right).
Flow Cytometry - Recombinant Human IFN gamma Protein (A330762)
Flow cytometry analysis of NCI-H460 cells (1X10^6) treated with Recombinant Human IFN gamma Protein (A330762) at 1 ng/mL stained with PE Rabbit IgG isotype control at 5 µl/Test (left) or PE Rabbit anti-Human PD-L1/CD274 mAb at 5 µl/Test (right)
