Stimulation of the p70 S6 Kinase (Thr389) and p85 S6 Kinase (Thr412) autophosphorylation in 293T human embryonic kidney cells: 0.01-1 ng/mL. Immobilized recombinant human IGFBP6 at 1 µg/mL bind recombinant human IGF1 in a functional ELISA with a linear range of 30-250 ng/mL. Cell proliferation assay using MCF-7 cells: ED50 = typically 7.5-30 ng/mL; Specific activity =3.33×10^4-1.33×10^5units/mg.
Purity
>87% (by SDS-PAGE).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).
Endotoxin Level
<0.1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Western Blot - Recombinant Human IGF1 Protein (C-terminal Human Fc and His Tag) (A330770)
p70S6 Kinase(Thr389) and p85 S6 Kinase(Thr412) autophosphorylation in 293T Human embryonic kidney cells analyzed by western blot. Primary antibody incubation was performed with Recombinant Human IGF1 Protein (C-terminal Human Fc and His Tag) (A330770) at 0.01-1ng/mL and detected with chemiluminescence.
Standard Curve - Recombinant Human IGF1 Protein (C-terminal Human Fc and His Tag) (A330770)
ELISA examination of immobilized recombinant human IGFBP6 (1µg/mL) binding Recombinant Human IGF1 Protein (C-terminal Human Fc and His Tag) (A330770): linear range = 30-250ng/mL.
Recombinant Human IGF1 Protein (C-terminal Human Fc and His Tag) (A330770)
Cell proliferation assay using Recombinant Human IGF1 Protein (C-terminal Human Fc and His Tag) (A330770) on MCF-7 cells : ED50 = typically 7.5-30 ng/mL.
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