Assay 1: The biological activity was measured by its binding ability in a functional ELISA, the ED50 is 0.5-5 µg/ml. Assay 2: The biological activity was determined by the dose-dependent stimulation of the proliferation of mouse D10S cells, the ED50 is <1pg/ml, corresponding to a specific activity of >1x109 U/mg.
Purity
>97% as determined by SDS-PAGE.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from 20 mM Phosphate Buffered Saline, pH 7.4, with 10% Trehalose.
Storage
Shipped at ambient temperature. Lyophilized: Short term (3 weeks) store at 25°C. Long term store desiccated below -18°C. Reconstituted: Short term (2-7 days) store at 4°C. Long term store below -18°C, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid freeze/thaw cycles.
Synonyms
Hematopoietin-1, IL1A, IL1F1, Interleukin-1 alpha
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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