Inhibition of cell proliferation for MC/9-2 mouse mast cells: ED50 = 0.13-0.52ng/mL; Specific activity = 1.92×10^6-7.69×10^6units/mg. Immobilized Human IL10 (2µg/mL) bind Human IL10RA in a functional ELISA with a linear range of 0.1-2.9ng/mL.
Purity
>92% (by SDS-PAGE).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).
Endotoxin Level
<0.1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Recombinant Human IL-10 Protein (C-terminal His Tag) (A330804)
Cell proliferation assay using Recombinant Human IL-10 Protein (C-terminal His Tag) (A330804) on MC/9 2 Mouse mast cells : ED50 = 0.13-0.52 ng/mL; Specific activity = 1.92×10^6-7.69×10^6units/mg.
Standard Curve - Recombinant Human IL-10 Protein (C-terminal His Tag) (A330804)
ELISA examination of immobilized Human IL10 (2 µg/mL) binding Recombinant Human IL-10 Protein (C-terminal His Tag) (A330804): linear range = 0.1-2.9 ng/mL.
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