Immobilized Recombinant Human IL-12 (1 µg/mL) bind Recombinant Human IL12Rß1 in a functional ELISA with a linear range of 30.6-122.4 ng/mL. Cell proliferation assay using anti-CD3 antibody activated Human T lymphocyte leukemia cells (jurkat): ED50 = typically 0.1-0.4 ng/mL; Specific activity =2.5×10^6-1.0×10^7units/mg. Induction of the Interferon gamma secretion by human natural killer lymphoma NK-92 cells: ED50 = 2-8 ng/mL; Specific activity =1.25×10^5~5×10^5 units/mg.
Purity
>95% (by SDS-PAGE).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).
Endotoxin Level
<0.1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
SDS-PAGE - Recombinant Human IL-12A + IL-12B Protein (C-terminal His (IL12B) and Flag Tag (IL12A)) (A331659)
SDS-PAGE of Recombinant Human IL-12A + IL-12B Protein (C-terminal His (IL12B) and Flag Tag (IL12A)) (A331659) using Coomassie Blue to show bands at 37 kDa and 40-55 kDa.
Standard Curve - Recombinant Human IL-12A + IL-12B Protein (C-terminal His (IL12B) and Flag Tag (IL12A)) (A331659)
ELISA examination of immobilized Recombinant Human IL-12 (1µg/mL) binding Recombinant Human IL-12A + IL-12B Protein (C-terminal His (IL12B) and Flag Tag (IL12A)) (A331659): linear range = 30.6-122.4 ng/mL.
Recombinant Human IL-12A + IL-12B Protein (C-terminal His (IL12B) and Flag Tag (IL12A)) (A331659)
Cell proliferation assay using Recombinant Human IL-12A + IL-12B Protein (C-terminal His (IL12B) and Flag Tag (IL12A)) (A331659) on anti-CD3 antibody activated Human T lymphocyte leukemia cells (jurkat) : ED50 = typically 0.1-0.4 ng/mL; Specific activity = 2.5×10^6-1.0×10^7units/mg.
Standard Curve - Recombinant Human IL-12A + IL-12B Protein (C-terminal His (IL12B) and Flag Tag (IL12A)) (A331659)
ELISA examination of Recombinant Human IL-12A + IL-12B Protein (C-terminal His (IL12B) and Flag Tag (IL12A)) (A331659) to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells: ED50 = 2-8 ng/mL; Specific activity = 1.25×10^5~5×10^5 units/mg.
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