ELISA: 0.2 - 0.4ng/well, Functional Assays: 0.1ng/ml for 50 µg, 0.1 for 10 µg, WB: 1.5 - 3ng/lane
Expression System
Escherichia coli
Nature
Recombinant
Protein Species
Human
Predicted MW
15.4 kDa
Purity
>98% (by SDS-PAGE and HPLC).
Product Form
Lyophilized
Concentration
100 µg/ml after reconstitution.
Formulation
Supplied in 10mM Sodium Citrate.
Reconstitution
Pack Size of 50 µg: Reconstitute with 0.5 ml 0.1M acetic acid. Pack Size of 10 µg: Reconstitute with 0.1 ml 0.1M acetic acid. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution.
Endotoxin Level
< 0.1 ng/µg
Storage
Lyophilized: Store at -20°C. Reconstituted: Store undiluted at -20°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Recombinant human interleukin 2 is an Escherichia coli-derived recombinant protein corresponding to full-length mature human interleukin-2, a secreted peptide required for T-cell proliferation. This preparation has an ED50 of 0.1ng/ml as determined by the dose-dependent stimulation of the proliferation of murine CTLL-2 cells.
Synonyms
IL2, Interleukin-2, T-cell growth factor, TCGF
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Sandwich ELISA analysis of human interleukin-2 using Anti-IL-2 Antibody [MQ1-17H12] (A281261) as a capture reagent and Anti-IL-2 Antibody (Biotin) - BSA and Azide free (A282248) as a detection reagent with Recombinant Human IL-2 Protein (A319852) as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Microplate Absorbance Reader. In the enlargement, neat serum (orange) and neat plasma (green) samples, low levels of human IL-2 (~1pg/ml) are detected
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