ELISA: 0.2 - 0.4ng/well, Functional Assays: 0.1ng/ml for 50 µg, 0.1 for 10 µg, WB: 1.5 - 3ng/lane
Expression System
Escherichia coli
Nature
Recombinant
Protein Species
Human
Predicted MW
15.4 kDa
Purity
>98% (by SDS-PAGE and HPLC).
Product Form
Lyophilized
Concentration
100 µg/ml after reconstitution.
Formulation
Supplied in 10mM Sodium Citrate.
Endotoxin Level
< 0.1 ng/µg
Storage
Lyophilized: Store at -20°C. Reconstituted: Store undiluted at -20°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
Synonyms
IL2, Interleukin-2, T-cell growth factor, TCGF
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Sandwich ELISA analysis of human interleukin-2 using Anti-IL-2 Antibody [MQ1-17H12] (A281261) as a capture reagent and Anti-IL-2 Antibody (Biotin) - BSA and Azide free (A282248) as a detection reagent with Recombinant Human IL-2 Protein (A319852) as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Microplate Absorbance Reader. In the enlargement, neat serum (orange) and neat plasma (green) samples, low levels of human IL-2 (~1pg/ml) are detected
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