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Recombinant Human IL-27-A + EBI3 Protein (N-terminal His and NO Tag) (A330876)

SDS-PAGE - Recombinant Human IL-27-A + EBI3 Protein (N-terminal His and NO Tag) (A330876) - Antibodies.com Standard Curve - Recombinant Human IL-27-A + EBI3 Protein (N-terminal His and NO Tag) (A330876) - Antibodies.com SPR Assay - Recombinant Human IL-27-A + EBI3 Protein (N-terminal His and NO Tag) (A330876) - Antibodies.com
$320
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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Recombinant Human IL-27-A + EBI3 Protein (N-terminal His and NO Tag)
Expression System
HEK293 cells
Nature
Recombinant
Protein Species
Human
Sequence
PRPPGRPQLSLQELRREFTVSLHLARKLLSEVRGQAHRFAESHLPGVNLYLLPLGEQLPDVSLTFQAWRRLSDPERLCFISTTLQPFHALLGGLGTQGRWTNMERMQLWAMRLDLRDLQRHLRFQVLAAGFNLPEEEEEEEEEEEEERKGLLPGALGSALQGPAQVSWPQLLSTYRLLHSLELVLSRAVRELLLLSKAGHSVWPLGFPTLSPQP&RKGPPAALTLPRVQCRASRYPIAVDCSWTLPPAPNSTSPVSFIATYRLGMAARGHSWPCLQQTPTSTSCTITDVQLFSMAPYVLNVTAVHPWGSSSSFVPFITEHIIKPDPPEGVRLSPLAERQLQVQWEPPGSWPFPEIFSLKYWIRYKRQGAARFHRVGPIEATSFILRAVRPRARYYVQVAAQDLTDYGELSDWSLPATATMSLGK
Tag
N-terminal His and NO Tag
Conjugate

Unconjugated

Purity
>95% (by SDS-PAGE).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).
Endotoxin Level
<1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

SDS-PAGE - Recombinant Human IL-27-A + EBI3 Protein (N-terminal His and NO Tag) (A330876) - Antibodies.comEnlarge Image

SDS-PAGE - Recombinant Human IL-27-A + EBI3 Protein (N-terminal His and NO Tag) (A330876)

SDS-PAGE of Recombinant Human IL-27-A + EBI3 Protein (N-terminal His and NO Tag) (A330876) using Coomassie Blue to show a band at 25-35 kDa.
Standard Curve - Recombinant Human IL-27-A + EBI3 Protein (N-terminal His and NO Tag) (A330876) - Antibodies.comEnlarge Image

Standard Curve - Recombinant Human IL-27-A + EBI3 Protein (N-terminal His and NO Tag) (A330876)

ELISA examination of immobilized Human IL-27 RA, hFc Tag, (5 µg/mL) binding Recombinant Human IL-27-A + EBI3 Protein (N-terminal His and NO Tag) (A330876): EC50 =55.9ng/mL.
SPR Assay - Recombinant Human IL-27-A + EBI3 Protein (N-terminal His and NO Tag) (A330876) - Antibodies.comEnlarge Image

Recombinant Human IL-27-A + EBI3 Protein (N-terminal His and NO Tag) (A330876)

Recombinant Human IL-27-A + EBI3 Protein (N-terminal His and NO Tag) (A330876) binding to Human IL-27 RA (Human Fc Tag) on a CM5 Chip via Protein A, analyzed by surface plasmon resonance (SPR; Biacore T200). Affinity constant = 3.58 nM.
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