Immobilized Human IL6R (1 µg/mL) bind Human IL-6 in a functional ELISA with a linear range of 2-15 ng/mL. Cell proliferation assay using TF-1 human erythroleukemic cells: ED50 = 0.07-0.26 ng/mL; Specific activity =3.85 × 10^6~1.43 × 10^7 units/mg.
Purity
>95% (by SDS-PAGE).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).
Endotoxin Level
<0.1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Standard Curve - Recombinant Human IL-6 Protein (C-terminal His Tag) (A330919)
ELISA examination of immobilized Human IL6R (1 µg/mL) binding Recombinant Human IL-6 Protein (C-terminal His Tag) (A330919): linear range = 2-15 ng/mL.
Recombinant Human IL-6 Protein (C-terminal His Tag) (A330919)
Cell proliferation assay using Recombinant Human IL-6 Protein (C-terminal His Tag) (A330919) on TF-1 Human erythroleukemic cells : ED50 = 0.07-0.26 ng/mL; Specific activity = 3.85 × 10^6~1.43 × 10^7 units/mg.
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