Immobilized Human IL7 (2 µg/mL) bind human IL7R in a functional ELISA with a linear range of 0.97 -159.1 ng/mL. Cell proliferation assay using IL-2-activated mouse spleen cell: ED50 = 0.1-0.4 ng/mL; Specific activity =2.5×10^6~1×10^7 units/mg. Cell proliferation assay using murine 2E8 cells: ED50 = 0.78-3.1ng/mL; Specific activity =3.23×10^5~1.28×10^6 units/mg.
Purity
>90% (by SDS-PAGE).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).
Endotoxin Level
<0.1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Synonyms
IL7, Interleukin-7
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Standard Curve - Recombinant Human IL-7 Protein (C-terminal His Tag) (A330925)
ELISA examination of immobilized Human IL7 (2 µg/mL) binding Recombinant Human IL-7 Protein (C-terminal His Tag) (A330925): linear range = 0.97 -159.1 ng/mL.
Recombinant Human IL-7 Protein (C-terminal His Tag) (A330925)
Cell proliferation assay using Recombinant Human IL-7 Protein (C-terminal His Tag) (A330925) on IL-2-activated mouse spleen cell : ED50 = 0.1-0.4 ng/mL; Specific activity = 2.5×10^6~1×10^7 units/mg.
Recombinant Human IL-7 Protein (C-terminal His Tag) (A330925)
Cell proliferation assay using Recombinant Human IL-7 Protein (C-terminal His Tag) (A330925) on Murine 2E8 cells : ED50 = 0.78-3.1ng/mL; Specific activity = 3.23×10^5~1.28×10^6 units/mg.
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