Recombinant Human Intestinal Alkaline Phosphatase Protein (N-terminal His and Flag Tag) (A330951)

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This product is discontinued

Recombinant Human Intestinal Alkaline Phosphatase Protein (N-terminal His and Flag Tag) (A330951) has been discontinued and is no longer available.

View all Intestinal Alkaline Phosphatase Proteins.

Specifications

Name

Recombinant Human Intestinal Alkaline Phosphatase Protein (N-terminal His and Flag Tag)

Expression System

HEK293 cells

Nature

Recombinant

Protein Species

Human

Sequence

VIPAEEENPAFWNRQAAEALDAAKKLQPIQKVAKNLILFLGDGLGVPTVTATRILKGQKNGKLGPETPLAMDRFPYLALSKTYNVDRQVPDSAATATAYLCGVKANFQTIGLSAAARFNQCNTTRGNEVISVMNRAKQAGKSVGVVTTTRVQHASPAGTYAHTVNRNWYSDADMPASARQEGCQDIATQLISNMDIDVILGGGRKYMFPMGTPDPEYPADASQNGIRLDGKNLVQEWLAKHQGAWYVWNRTELMQASLDQSVTHLMGLFEPGDTKYEIHRDPTLDPSLMEMTEAALRLLSRNPRGFYLFVEGGRIDHGHHEGVAYQALTEAVMFDDAIERAGQLTSEEDTLTLVTADHSHVFSFGGYTLRGSSIFGLAPSKAQDSKAYTSILYGNGPGYVFNSGVRPDVNESESGSPDYQQQAAVPLSSETHGGEDVAVFARGPQAHLVHGVQEQSFVAHVMAFAACLEPYTACDLAPPACTTD

Tag

N-terminal His and Flag Tag

Conjugate

Unconjugated

Purity

>95% (by Tris-Bis PAGE and HPLC).

Product Form

Lyophilized

Concentration

Reconstitution dependent.

Endotoxin Level

<1 EU/µg of the protein by LAL method.

Storage

Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.

Synonyms

ALPI, IAP, Intestinal-type alkaline phosphatase

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Recombinant Human Intestinal Alkaline Phosphatase Protein (N-terminal His and Flag Tag) (A330951)

Tris-Bis PAGE of Recombinant Human Intestinal Alkaline Phosphatase Protein (N-terminal His and Flag Tag) (A330951) under reducing conditions confirms that purity is greater than 95%.

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Recombinant Human Intestinal Alkaline Phosphatase Protein (N-terminal His and Flag Tag) (A330951)

SEC-HPLC analysis of Recombinant Human Intestinal Alkaline Phosphatase Protein (N-terminal His and Flag Tag) (A330951) confirms that purity is greater than 95%.

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Recombinant Human Intestinal Alkaline Phosphatase Protein (N-terminal His and Flag Tag) (A330951)

Recombinant Human Intestinal Alkaline Phosphatase Protein (N-terminal His and Flag Tag) (A330951) binding to Anti-Alkaline Phosphatase Antibody on a CM5 Chip via Protein A, analyzed by surface plasmon resonance (SPR; Biacore T200). Affinity constant = 0.96 µM.