Recombinant Human Leptin Triple Antagonist Protein (N-terminal PEG Tag)
Applications
Cell Proliferation Assay
Expression System
Escherichia coli
Nature
Recombinant
Protein Species
Human
Tag
N-terminal PEG Tag
Predicted MW
35.6 kDa
Biologically Active
Yes
Biological Activity
Capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. Its in vitro activity is 6-8 fold lower than the non-pegylated antagonist but in vivo it has profound weight gain effect (as compared to the non-pegylated antagonist), resulting mainly from increased food intake. Its in vivo activity compared to that of PEG-MLA is 9-27 fold higher.
Purity
>98% as determined by Gel Filtration and SDS-PAGE.
Product Form
Lyophilized
Concentration
0.65 mg/ml
Formulation
Lyophilized from a solution containing 3 µM Sodium Bicarbonate.
Storage
Shipped at ambient temperature. Lyophilized: Short term (several weeks) store at 25°C. Long term store desiccated below -20°C. Reconstituted: Short term (several weeks) store at 4°C or 25°C. At lower concentrations it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid freeze/thaw cycles.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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