Immobilized Human CSF1 (1µg/mL) bind Human CSF1R in a functional ELISA with a linear range of 0.01-7.8 ng/mL. Cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells: ED50 = typically 1.53-6.14 ng/mL; Specific activity =1.63×10^5-6.53×10^5units/mg.
Purity
>95% (by SDS-PAGE).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).
Endotoxin Level
<0.1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Recombinant Human M-CSF Protein (C-terminal His Tag) (A331043)
Cell proliferation assay using Recombinant Human M-CSF Protein (C-terminal His Tag) (A331043) on M-NFS-60 Mouse myelogenous leukemia lymphoblast cells : ED50 = typically 1.53-6.14 ng/mL; Specific activity = 1.63×10^5-6.53×10^5units/mg.
Standard Curve - Recombinant Human M-CSF Protein (C-terminal His Tag) (A331043)
ELISA examination of immobilized Human CSF1 (1µg/mL) binding Recombinant Human M-CSF Protein (C-terminal His Tag) (A331043): linear range = 0.01-7.8 ng/mL.
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