Reconstitute with 0.2 ml of distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution.
Endotoxin Level
< 0.1 ng/µg
Storage
Lyophilized: Store at -20°C. Reconstituted: Store undiluted at -20°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Recombinant human MCP-1/MCAF also known as C-C motif chemokine 2, Monocyte chemoattractant protein 1, Monocyte chemoattractant protein 1, HC11 or Small-inducible cytokine A2 is encoded by the CCL2 gene. Recombinant MCP-1 is a 76 amino acid ~13kDa chemokine involved in chemotaxis and polarization of the immune response.Recombinant human MCP-1/MCAF has been employed successfully as a control antigen in the development of a sensitive ELISA employing Mouse anti Human MCP-1 antibody clone 2.2-4A4-1A11 as a capture reagent and biotinylated Rabbit anti Human MCP-1 polyclonal antibody (AHP784B) as a detection reagent.
Synonyms
C-C motif chemokine 2, CCL2, HC11, MCAF, MCP-1, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, Monocyte chemotactic protein 1, Monocyte secretory protein JE, SCYA2, Small-inducible cytokine A2
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Sandwich ELISA analysis of human MCP-1 using Anti-MCP1 Antibody [2.2-4H5-1A11] - BSA and Azide free (A279120) as a capture reagent and Anti-MCP1 Antibody (Biotin) - BSA and Azide free (A282353) as a detection reagent with Recombinant Human MCP1 Protein (A320413) as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Microplate Absorbance Reader. Serum (green) and plasma (orange) samples are included undiluted.
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