~125-175 U/mg, also phosphorylates ERK1, ERK2, and GSK-3.
Unit Definition
One unit is defined as the transfer of 1 nmol of phosphate to ERK1/2 peptide per minute at 30°C in a reaction containing 100µM ATP.
Purity
>90% as determined by SDS-PAGE.
Product Form
Liquid
Concentration
Lot Specific
Formulation
Supplied in 40 mM Tris Buffer, pH 8, with 150 mM Sodium Chloride, 270 mM Sucrose, 1 mM DTT, 200 µM PMSF, 1 mM Benzamidine, 100 µM Sodium vanadate, and 0.03% Brij-35.
Storage
Shipped at 4°C. Short term (1-2 weeks) store at 4°C. Long term store at -20°C. Avoid freeze/thaw cycles.
