Recombinant Human NF-L Protein (A270573)

Coomassie brilliant blue stained SDS-PAGE gel of Recombinant Human NF-L Protein (A270573) and various other full length recombinant human proteins. The lanes contain: [Lane 1] protein standards, [Lane 2] 1 µg of Recombinant Human Peripherin Protein (A270574), [Lane 3] 1 µg of Recombinant Human alpha Internexin Protein (A270567), [Lane 4] 1 µg of Recombinant Human Vimentin Protein (A270578), and [Lane 5] 1 µg of Recombinant Human NF-L Protein (A270573). In each case the proteins run approximately 5 kDa slower than the native protein due to the addition of the His-tag and other vector-derived sequences.

Western blots of Uman NF-LIGHT™ antibodies and a set of reagents on Recombinant Human NF-L Protein (A270573) and Recombinant Human NF L Protein (A333288). The lanes contain: [Lane 1] protein standard (red), [Lane 2] Recombinant Human NF-L Protein (A270573), [Lane 3] Recombinant Human NF L Protein (A333288). Recombinant Human NF-L Protein (A270573) runs at about 75kDa, while Recombinant Human NF L Protein (A333288) runs at about 12kDa. All five antibodies recognize both constructs. UD1 is also known as 2.1 is the detection reagent in the Uman NF-LIGHT™ assay while UD2, also known as 47.3 is the capture reagent (6). The three other lanes show results obtained with Anti-NF-L Antibody [1B11] (A104310) and Anti-NF-L Antibody [6H63] (A333276) respectively as indicated. All these antibodies binds to an epitope flanking the so-called second “stutter” in the Coil 2 region of the a-helical coiled coil “rod” region of NF-L. Anti-NF-L Antibody [6H63] (A333276) binds an epitope just N-terminal to the Uman antibody UD2.