Immobilized Human BMP2 (1 µg/mL) bind Noggin in a functional ELISA with a linear range of 2-57 ng/mL. Immobilized Human BMP4 (0.5 µg/mL) bind Noggin in a functional ELISA with a linear range of 4-47 ng/mL. Immobilized Human Noggin (1 µg/mL) bind Noggin Rabbit pAb in a functional ELISA with a linear range of 1-3.5 ng/mL. Inhibition of the BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells: ED50 = 13.28-53.12 ng/mL in the presence of 50 ng/mL of recombinant human BMP-4; Specific activity =1.88×10^4-7.53×10^4 units/mg.
Purity
>95% (by SDS-PAGE).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).
Endotoxin Level
<0.1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Synonyms
NOG
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Standard Curve - Recombinant Human Noggin Protein (C-terminal His Tag) (A331113)
ELISA examination of immobilized recombinant Human BMP2 (1 µg/mL) binding Recombinant Human Noggin Protein (C-terminal His Tag) (A331113): linear range = 2-57 ng/mL.
Standard Curve - Recombinant Human Noggin Protein (C-terminal His Tag) (A331113)
ELISA examination of immobilized recombinant Human BMP4 (0.5 µg/mL) binding Recombinant Human Noggin Protein (C-terminal His Tag) (A331113): linear range = 4-47 ng/mL.
Standard Curve - Recombinant Human Noggin Protein (C-terminal His Tag) (A331113)
ELISA examination of immobilized Human Noggin (1 µg/mL) binding Recombinant Human Noggin Protein (C-terminal His Tag) (A331113): linear range = 1-3.5 ng/mL.
Standard Curve - Recombinant Human Noggin Protein (C-terminal His Tag) (A331113)
ELISA examination of Recombinant Human Noggin Protein (C-terminal His Tag) (A331113) to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells: ED50 = 13.28-53.12 ng/mL in the presence of recombinant human BMP-4 (50 ng/mL); Specific activity = 1.88×10^4-7.53×10^4 units/mg.
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