Serum-free cell proliferation assay using MCF-7 human breast cancer cells: ED50 = typically 6.2-24.7ng/mL; Specific activity = 4.0×10^4-1.61×10^5units/mg.
Purity
>95% (by SDS-PAGE).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from 20mM Tris, pH 8.0, with 150mM NaCl and 5% Glycerol (0.22µm filter sterilized).
Endotoxin Level
<1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Cell proliferation assay using Recombinant Human NRG1 Protein (A331118) on MCF-7 Human breast cancer cells : ED50 = typically 6.2-24.7 ng/mL; Specific activity = 4.0×10^4-1.61×10^5units/mg.
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