Immobilized Human SPP1 Protein (1 µg/mL) bind Osteopontin Rabbit mAb in a functional ELISA with a linear range of 0.976-11.58 ng/mL.
Purity
>87% (by SDS-PAGE).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).
Endotoxin Level
<0.1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Synonyms
BNSP, Bone sialoprotein 1, Nephropontin, OPN, Secreted phosphoprotein 1, SPP-1, SPP1, Urinary stone protein, Uropontin
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Standard Curve - Recombinant Human Osteopontin Protein (C-terminal His Tag) (A331131)
ELISA examination of immobilized recombinant Human SPP1 Protein (1µg/mL) binding Recombinant Human Osteopontin Protein (C-terminal His Tag) (A331131): linear range = 0.976-11.58ng/mL.
Standard Curve - Recombinant Human Osteopontin Protein (C-terminal His Tag) (A331131)
ELISA examination of immobilized Recombinant Human Osteopontin Protein (C-terminal His Tag) (A331131) to support the adhesion of B16 cells (5×10^4 cells/well): ED50 = 23.9-95.7 ng/mL after 1 hour incubation at 37 °C.
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