Recombinant Human P4HB Protein (N-terminal His Tag) (A62983)

0.001 650nm/min-2. By measuring the turbidity increase at 650 nm due to insulin reduction (Holmgren, A. (1979) J. Biol. Chem. 254, 9627-9632). The activity is expressed as the ratio of the slope of a linear part of the turbidity curve to the lag time (Mart?´nez-Galisteo, E., Padilla, C. A., Garcia-Alfonso, C., Lo´pez-Barea, J., and Barcena, J. A. (1993) Biochimie (Paris) 75, 803-809). 0.5 µmol active RNase A min-1 µmol PDI-1. According to the re-activation of reduced and denatured RNase A (Lyles, M. M. and Gilbert, H. F. (1991) Biochemistry 30, 613-619).

>95% as determined by RP-HPLC and SDS-PAGE.

Lyophilized

1 mg/ml

Lyophilized from Phosphate Buffered Saline, pH 7.

Shipped at ambient temperature. Lyophilized: Short term (3 weeks) store at 25°C. Long term store desiccated below -18°C. Reconstituted: Short term (2-7 days) store at 4°C. Long term store below -18°C, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid freeze/thaw cycles.

Cellular thyroid hormone-binding protein, ERBA2L, p55, PDI, PDIA1, PO4DB, Prolyl 4-hydroxylase subunit beta, Protein disulfide-isomerase