Immobilized Human TNFSF11 Protein (2 µg/mL) bind Mouse TNFRSF11B in a functional ELISA with a linear range of 0.049-13.74 ng/mL. Induction of the osteoclast differentiation of RAW 264.7 mouse monocyte/macrophage cells: ED50 = 10.85-43.42 ng/mL; Specific activity =2.30×104~9.22×104 units/mg.
Purity
>90% (by SDS-PAGE).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).
Endotoxin Level
<0.1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Standard Curve - Recombinant Human RANKL Protein (N-terminal His Tag) (A331210)
ELISA examination of immobilized Human TNFSF11 Protein (2 µg/mL) binding Recombinant Human RANKL Protein (N-terminal His Tag) (A331210): linear range = 0.049-13.74 ng/mL.
Recombinant Human RANKL Protein (N-terminal His Tag) (A331210)
Osteoclast differentiation of RAW 264.7 Mouse monocyte/macrophage cells induced by Recombinant Human RANKL Protein (N-terminal His Tag) (A331210): ED50 = 10.85-43.42 ng/mL; Specific activity = 2.30×10^4~9.22×10^4 units/mg.
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