Ability to enhance Cyclin D1 expression in HCT116 human colon adenocarcinoma cells: 0.1-10ng/mL. Intestinal crypts of mice were cultured in organoid culture medium containing factor combinations (100ng/mL Noggin, 500ng/mL R-spindin-1) for 144 hours, intestinal organoids were formed. Stimulation of Wnt signal pathway with Wnt-3a protein in HEK293T cells: 300ng/mL after 6 hours compared to Wnt-3a stimulation after 300ng/mL.
Purity
>95% (by SDS-PAGE).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).
Endotoxin Level
<0.1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Western Blot - Recombinant Human RSPO1 Protein (C-terminal His Tag) (A331229)
Cyclin D1 expression in HCT116 Human colon adenocarcinoma cells analyzed by western blot. Primary antibody incubation was performed with Recombinant Human RSPO1 Protein (C-terminal His Tag) (A331229) at 0.01-1ng/mL and detected with chemiluminescence.
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