Inhibition of Anti-CD3-induced proliferation of jurkat cells: ED50 = 1.8-7.2µg/mL.
Purity
>95% (by SDS-PAGE).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from Phosphate Buffered Saline, pH 7.4, with 300mM NaCl and without preservatives or carriers (0.22µm filter sterilized).
Reconstitution
Centrifuge the vial before opening and reconstitute with distilled sterile water to a concentration of 0.1-0.5 mg/mL. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer.
Endotoxin Level
<0.1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Recombinant Human SIGLEC15 Protein (C-terminal Human Fc and His Tag) (A331260)
Inhibition of Anti-CD3-induced cell proliferation for Jurkat cells with Recombinant Human SIGLEC15 Protein (C-terminal Human Fc and His Tag) (A331260): ED50 = 1.8-7.2 µg/mL.
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