Recombinant Human SIRP alpha Protein (C-terminal Human Fc and His Tag) (A331271)

Name: Recombinant Human SIRP alpha Protein (C-terminal Human Fc and His Tag)
Brand: Antibodies.com
SKU: A331271
Price: 115.0 GBP
Availability: InStock

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$165

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Recombinant Human SIRP alpha Protein (C-terminal Human Fc and His Tag)

Expression System

HEK293 cells

Nature

Recombinant

Protein Species

Human

Sequence

EEELQVIQPDKSVLVAAGETATLRCTATSLIPVGPIQWFRGAGPGRELIYNQKEGHFPRVTTVSDLTKRNNMDFSIRIGNITPADAGTYYCVKFRKGSPDDVEFKSGAGTELSVRAKPSAPVVSGPAARATPQHTVSFTCESHGFSPRDITLKWFKNGNELSDFQTNVDPVGESVSYSIHSTAKVVLTREDVHSQVICEVAHVTLQGDPLRGTANLSETIRVPPTLEVTQQPVRAENQVNVTCQVRKFYPQRLQLTWLENGNVSRTETASTVTENKDGTYNWMSWLLVNVSAHRDDVKLTCQVEHDGQPAVSKSHDLKVSAHPKEQGSNTAAENTGSNER

Tag

C-terminal Human Fc and His Tag

Conjugate

Unconjugated

Biological Activity

Immobilized Recombinant Human CD47 (1 µg/mL) bind Recombinant Human SIRP alpha in a functional ELISA with a linear range of 1-6 ng/mL. Loaded Human SIRP-alpha/CD172a Protein, C-terminal Human Fc and Hison Tag, ProA Biosensor, can bind Human CD47 Protein, C-terminal His Tag, in BLI assay (Gator) with an affinity constant of 11.1 nM. Immobilized protein to support the adhesion of the OVCAR-3 cells: ED50 = 0.53-2.12 µg/mL.

Purity

>95% (by SDS-PAGE).

Product Form

Lyophilized

Concentration

Reconstitution dependent.

Formulation

Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).

Endotoxin Level

<0.1 EU/µg of the protein by LAL method.

Storage

Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.

Synonyms

BIT, Brain Ig-like molecule with tyrosine-based activation motifs, CD172 antigen-like family member A, CD172a, Inhibitory receptor SHPS-1, Macrophage fusion receptor, MFR, MyD-1 antigen, MYD1, PTPNS1, SHP substrate 1, SHPS-1, SHPS1, SIRP, SIRPA, Tyrosine-protein phosphatase non-receptor type substrate 1

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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SDS-PAGE - Recombinant Human SIRP alpha Protein (C-terminal Human Fc and His Tag) (A331271)

SDS-PAGE of Recombinant Human SIRP alpha Protein (C-terminal Human Fc and His Tag) (A331271) using Coomassie Blue to show a band at 80-100 kDa.

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Recombinant Human SIRP alpha Protein (C-terminal Human Fc and His Tag) (A331271)

SEC-HPLC analysis of Recombinant Human SIRP alpha Protein (C-terminal Human Fc and His Tag) (A331271) confirms that purity is greater than 90%.

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Standard Curve - Recombinant Human SIRP alpha Protein (C-terminal Human Fc and His Tag) (A331271)

ELISA examination of immobilized Recombinant Human CD47 (1 µg/mL) binding Recombinant Human SIRP alpha Protein (C-terminal Human Fc and His Tag) (A331271): linear range = 1-6 ng/mL.

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Recombinant Human SIRP alpha Protein (C-terminal Human Fc and His Tag) (A331271)

Human CD47 Protein, C-His Tag, binding to Recombinant Human SIRP alpha Protein (C-terminal Human Fc and His Tag) (A331271) immobilized on ProA Biosensor, analyzed by bio-layer interferometry (BLI; Gator). Affinity constant = 11.1 nM.

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