Inhibition of p53 expression in C3H10T1/2 mouse embryonic fibroblast cells: 1.25-2.5µg/mL.
Purity
>97% (by SDS-PAGE).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from 20mM Tris, pH 7.4, with 300mM NaCl and without preservatives or carriers (0.22µm filter sterilized).
Endotoxin Level
<1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Synonyms
HHG-1, SHH, Shh unprocessed N-terminal signaling and C-terminal autoprocessing domains, ShhNC, Sonic hedgehog protein
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Recombinant Human Sonic Hedgehog Protein (A331290)
p53 expression inhibition in C3H10T1/2 Mouse embryonic fibroblast cells analyzed by western blot. Primary antibody incubation was performed with Recombinant Human Sonic Hedgehog Protein (A331290) at 1.25-2.5µg/mL and detected with chemiluminescence.
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