Inhibition of the IL-4-dependent proliferation of HT-2 mouse T cells: ED50 = 24.2-96.6 pg/mL; Specific activity = 1.04×10^7~4.17×10^7 units/mg.
Purity
>95% (by SDS-PAGE).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from 50mM Glycine-HCl, pH 2.5, with 150mM NaCl (0.2µm filter sterilized).
Endotoxin Level
<1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
SDS-PAGE - Recombinant Human TGF beta 3 Protein (A331317)
SDS-PAGE of Recombinant Human TGF beta 3 Protein (A331317) under reducing (R) and non-reducing (NR) conditions to show bands at 13-16 kDa and 25-30 kDa, respectively.
Inhibition of IL-4-dependent cell proliferation for HT-2 mouse T cells with Recombinant Human TGF beta 3 Protein (A331317): ED50 = 24.2-96.6 pg/mL; Specific activity = 1.04×10^7~4.17×10^7 units/mg.
Western Blot - Recombinant Human TGF beta 3 Protein (A331317)
a-SMA and BMP7 expression in NRK-49F Rat kidney cells analyzed by western blot. Primary antibody incubation was performed with Recombinant Human TGF beta 3 Protein (A331317) at 0.5-5ng/mL and detected with chemiluminescence.
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