Immobilized Human TIMP1 Protein (1 µg/mL) bind TIMP1 Rabbit mAb in a functional ELISA with a linear range of 0.038-2.5 ng/mL. Inhibition of the human MMP-2 cleavage of a fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2: IC50 = approximately 2.2 nM.
Purity
>95% (by SDS-PAGE).
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).
Endotoxin Level
<1 EU/µg of the protein by LAL method.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C up to 1 year from the date of receipt. Reconstituted: Aliquot and store at -20°C for 3 months or 2-8°C for up to 1 week. Avoid freeze/thaw cycles.
Synonyms
CLGI, Collagenase inhibitor, EPA, Erythroid-potentiating activity, Fibroblast collagenase inhibitor, Metalloproteinase inhibitor 1, TIMP, TIMP-1, Tissue inhibitor of metalloproteinases 1
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Standard Curve - Recombinant Human TIMP1 Protein (C-terminal His Tag) (A331333)
ELISA examination of immobilized recombinant Human TIMP1 Protein (1µg/mL) binding Recombinant Human TIMP1 Protein (C-terminal His Tag) (A331333): linear range = 0.038-2.5ng/mL.
Recombinant Human TIMP1 Protein (C-terminal His Tag) (A331333)
Recombinant Human TIMP1 Protein (C-terminal His Tag) (A331333) inhibits human MMP-2 cleavage of a fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2: IC50 = approximately 2.2 nM.
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